LACK OF EFFECTS OF BACILLUS SPHAERICUS (VECTOLEX®) ON NONTARGET ORGANISMS IN A MOSQUITO-CONTROL PROGRAM IN SOUTHEASTERN WISCONSIN: A 3-YEAR STUDY

Author(s):  
Richard W. Merritt ◽  
Joanna L. Lessard ◽  
Kelly J. Wessell ◽  
Osvaldo Hernandez ◽  
Martin B. Berg ◽  
...  
2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Nor Shaida Husna Zulkrnin ◽  
Nurul Nadiah Rozhan ◽  
Nur Amanina Zulkfili ◽  
Nik Raihan Nik Yusoff ◽  
Mohd Sukhairi Mat Rasat ◽  
...  

Dengue is vector-borne diseases with 390 million infections per year extending over 120 countries of the world. Aedes aegypti (L.) (Diptera: Culicidae) is a primary vector for dengue viral infections for humans. Current focus on application of natural product against mosquito vectors has been the main priority for research due to its eco-safety. The extensive use of chemical insecticides has led to severe health problems, environmental pollution, toxic hazards to human and nontarget species, and development of insecticide resistance on mosquitoes. Azolla pinnata is an aquatic fern and predominantly used as feed in poultry industry and as fertilizer in agricultural field for enhancing the fertility of rice paddy soil. The present study was conducted to explore the larvicidal efficacy of A. pinnata using fresh and powdered form against late third-stage larvae (6 days, 5 mm in larvae body length) of Ae. aegypti (L.) (Diptera: Culicidae). The larvicidal bioassays were performed using World Health Organization standard larval susceptibility test method for different concentration for powdered and fresh A. pinnata. Powdered A. pinnata concentration used during larvicidal bioassay ranges from 500ppm to 2000ppm; meanwhile, fresh A. pinnata ranges from 500ppm to 9,000,000 ppm. The highest mortality was at 1853 ppm for powdered A. pinnata compared with fresh A. pinnata at 2,521,535 ppm, while the LC50 for both powdered and fresh A. pinnata recorded at 1262 ppm and 1853 ppm, respectively. Finally, the analysis of variance (ANOVA) showed significant difference on Ae. aegypti larval mortality (F=30.439, df=1, p≤0.001) and concentration (F=20.002, df=1, p≤0.001) compared to powdered and fresh A. pinnata at 24-hour bioassay test. In conclusion, the powdered A. pinnata serves as a good larvicidal agent against Ae. aegypti (L.) (Diptera: Culicidae) and this study provided information on the lethal concentration that may have potential for a more eco-friendly Aedes mosquito control program.


2019 ◽  
Vol 98 (8) ◽  
pp. 893-896
Author(s):  
Svetlana A. Roslavtseva

Mosquito control is necessary to improve the epidemic and, consequently, the sanitary and hygienic situation in human settlements. At the same time, the safest and more environmentally friendly way of controlling is not the fight against adult mosquitoes, but the treatment of reservoirs with microbiological larvicides based on entomopathogenic, aerobic, spore-forming, saprophytic bacteria Bacillus thuringiensis (de Barjac) (Bti). A new serotype of the bacterium B. thuringiensis was found in Israel in the Negev desert. This serotype being more active against larvae of blood-sucking and non-blood-sucking mosquitoes and midges than previously known serotypes, was named israelensis. Bti endotoxin is a typical insecticide with intestinal type of action for different mosquito species. For example, Bti H14 is highly insecticidal to the larvae of Aedes aegypti and Ae. albopictus at very low concentrations. The parasporal body (endotoxin crystal), a crystalline protein consisted of four main polypeptides and two minor polypeptides, possesses of a larvicidal action. Larvicidal activity is associated with a synergistic effect in a combination of four polypeptides. The possibility of development of resistance to products based on Bti and Bacillus sphaericus in populations of mosquitoes (Culicidae) was investigated. The use of domestic microbiological formulations based on Bti («Baktitsid», «Larviol-pasta», and «Antinat») was shown an eradication the larvae of bloodsucking mosquitoes and midges to be possible and rational, since they are not generated resistant populations of mosquitoes. This is confirmed by more than 30 years of the use of such formulations.


2020 ◽  
Author(s):  
Naiyana Sahavechaphan ◽  
Asamaporn Chatrattikorn ◽  
Pongsakorn Sadakorn ◽  
Darin Areechokechai ◽  
Sopon Iamsirithaworn

Abstract Background: The strategy for prevention and control of Aedes-borne diseases relies on timely elimination of key breeding containers. There is thus a crucial need to identify key breeding containers to enhance vector control activities. The visual larval survey of wet containers has then been conducted as a routine mission of the Department of Disease Control (DDC). To facilitate this, DDC has deployed a mobile application, namely TanRabad SURVEY, since May 2016. As per an inspected place, TanRabad SURVEY supports the real-time collection of its larval survey data and processing of its larval indices and key breeding containers. Methods: Larval survey data from 2017 to 2019 were spatially and temporally collected by public health officials via TanRabad SURVEY. Several measurements were computed to identify the overall and regional key breeding containers and places; and the regional transmission potentiality of Aedes-borne diseases. The measurements for identifying essential containers were breeding potentiality, productivity, contribution and preference ratio. As for places, the larval productivity of places based on larval indices was employed. Such place productivity was also implemented for discovering the regional transmission potentiality of Aedes-borne diseases. Results: The overall essential breeding containers were water tank, unused containers, other used containers, old tyres, anti-ant bowls, drip tray of water dispensers, pet bowls and plant leaves. As for regional aspects, different regions had different essential breeding containers wherein their most common containers were unused containers, other used containers, old tyres and drip tray of water dispensers. All regions had similar experiences that villages and temples were the top two riskiest places, followed by schools, factories, hotels and hospitals. Additionally, all regions had high transmission potentiality of Aedes-borne disease as above 30 of all their places were moderate and high risk places. Conclusions: This study identified the overall and regional key breeding containers and places along with the regional transmission potentiality of Aedes-borne diseases. Beside this, the empirical evidence had shown that the breeding productivity of most containers and places in each type was gradually decreased from time to time. This was mainly because TanRabad SURVEY supported real-time risk communication to community participants and effective prevention and control program development.


2018 ◽  
Vol 45 (2) ◽  
pp. 111-122 ◽  
Author(s):  
Afroza Sultana ◽  
Sharmin Hasan ◽  
Mosharraf Hossain ◽  
Abdul Alim ◽  
Mohammad Al Mamun ◽  
...  

Mosquito larval ecology is prerequisite for determining the larval abundance and species assemblage in mosquito control program. The study explored the association of five mosquito species with their breeding habitat diversity and species distribution in three selected parks from May to October, 2015. A total of 3217 mosquito larvae were reported from six breeding habitats, namely tree hole, leaf axils, water bottle, tire, drain and coconut shell. The frequency of the three species (Ae. aegypti, Ae. albopictus and Ar. subalbatus) coexistence was higher in tree holes (82.4%) than that of the other coexisting species. Pearson Chi-square result revealed that the association of species was significantly dependent on the breeding habitats. ANOVA further suggested that mosquito density varied across habitats where among the highest density of Cx. quinquefasciatus (3.87 ± 0.22) found in drain, followed by both Ae. albopictus (2.02 ± 0.17) and Ar. subalbatus (0.50 ± 0.09) in tree holes and Ae. aegypti (1.25 ± 0.23) in coconut shell. Cx. tritaeniorhynchus occurred in drain with the least observed density (0.03 ± 0.01). CCA results suggested that Aedes species were likely preferred to oviposit in shaded habitats where pH was associated with Ae. albopictus and dissolved oxygen was with Ae. aegypti and Ar. subalbatus. Culex species were positively associated with the habitats characterizing muddy bottom and emerging vegetation but not with any of the physico-chemical parameters. These findings concluded that ecological factors influence mosquito species to favor their breeding habitats can be helpful in controlling targeted vector species as well as the mosquito borne diseases.Bangladesh J. Zool. 45(2): 111-122, 2017


2018 ◽  
Vol 46 (4) ◽  
Author(s):  
Majematang Mading

Anopheles mosquitoes is main vector of malaria and filariasis. The use of bioinsecticide is an alternative way to reduce the negative impacts of synthetic insecticide. Areca catechu L. seeds contain secondary metabolites that have bioinsectisidal effects. The aim of this study was to analyze the effects of A.catechu L. seed extract on An. vagus larvae midgut. This study was a laboratory experimental research that designed as post test only control group design. A.catechu L. seed from Southwest Sumba Regency was extracted using ultrasonic methods. Larvae used in this study were Anopheles vagus instar III or early IV that devided into seven groups of the extract concetration (500; 1250; 2000; 2750; 3500; 4250 and 5000 ppm) and control group to find out the toxicity of the extract. Each group consists of twenty five larvae. The histopathology of larval midgut was stained with Hematoxyllin-Eosin and observed using light microscopy 24 hours after exposure. Histopathological examination of larval midgut showed the mild, moderate and severe damages on larval midgut after 24 hours exposure. Areca catechu L. seed extract can lead to changes in damage larval midgut. Areca catechu L. seed extract can be recommended to be used effectively as a natural larvicidal mosquito control program.


Author(s):  
Casey Parker ◽  
Felicita Garcia ◽  
Oscar Menocal ◽  
Dunia Jeer ◽  
Barry Alto

Dengue poses a significant public health threat and results in ~96 million clinical cases every year. Central America is a region burdened by neglected tropical diseases, including dengue. The primary vectors of dengue, Aedes aegypti and Aedes albopictus, are widely distributed in Honduras. Additionally, sustained and consistent mosquito control is lacking in the country. Successful control of container mosquitoes relies heavily on participation from community leaders, stakeholders, and the community itself. We conducted a pilot study in San Pedro Sula, Honduras where community leaders and stakeholders were trained on mosquito biology and control and were able to apply that knowledge to an underserved community in San Pedro Sula. Surveys to assess the number and type of containers in the community and the number of containers on the residence identified associations with select socioeconomic factors and other variables based on survey questions. The average number of containers on the premises was 15 (± 2.3) and the most prevalent containers (>50%) were flowerpots, garbage, and toys, which could be targeted in mosquito control programs. This pilot study offers a framework for training community leaders and stakeholders to create a sustainable community-based vector control program for container mosquitoes.


1976 ◽  
Vol 54 (3-4) ◽  
pp. 177-190 ◽  
Author(s):  
Gisèle Lamoureux ◽  
Estelle Lacoursière

The prime objective of a mosquito control that is based on the larvaciding program is the localization of breeding areas. French ecologists were the first to use the vegetation as an indicator of the mosquito breeding sites, and the success that has so far been incurred by this method encouraged its use for the mosquito control program of the 'Basse-Mauricie.' It is therefore with this in view that the authors undertook a study of vegetation groupings that characterized certain breeding sites. Field observations and analysis of phytosociological data permitted, within groupings of different water modes, the disclosure of a certain number of levels that constituted as many types of potential breeding sites.


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